| Human prolactin (hPRL) - Cat no. GHP-14 - GENE ID 5617 | |
| Description: | Recombinant human prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques |
| Source: | E.coli |
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| Physical Appearance: | White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized hPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. |
| Stability: | Lyophilized hPRL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 hPRL mg/ml and up to 4 mg/ml and filter sterilization hPRL can be stored at 4C for several weeks. |
| Purity: | Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. |
| Amino Acid Sequence: | The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Ile-Cys-Pro |
| Dimers and Aggregates: | The purified hPRL (23K) consists of > 99% monomers as determined by gel-filtration chromatography. |
| Biological Activity: | PLR’s hPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells. |
| Endotoxin: | Less than 0.1 ng/µg (IEU/µg) of hPRL |
| Protein content: | Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.92 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by theDNAman computer analysis program of protein sequences. |
| Usage: | This material is offered by PLR for laboratory research |
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