Protein Laboratories Rehovot Ltd.
December 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Mouse prolactin - Cat. no. GHP-25
Description: Recombinant mouse prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques

Source: E. coli
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Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.

Solubility: It is recommended to reconstitute the lyophilized mPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability: Lyophilized mPRL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 oPRL mg/ml and up to 4 mg/ml mM and filter sterilization oPRL can be stored at 4C for several weeks.

Purity: Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

Amino Acid Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Ile-Cys-Ser
Dimers and Aggregates: The purified mPRL (23K) consists of > 99% monomers as determined by gel-filtration chromatography.

Biological Activity: PLR’s mPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Endotoxin: Less than 0.05 ng/µg (0.5 IEU/µg) of mPRL 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.63 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.

Usage: This material is offered by PLR for laboratory research 
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