Protein Laboratories Rehovot Ltd.
October 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human interferon-alpha 2A - Cat. no. CYT-14
Description: IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase. Recombinant human interferon alpha 2A is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton according to the sequence of interferon-alpha 2A gene obtained from human leukocytes. The IFN-alpha 2A was purified by proprietary chromatographic techniques.
 
Source: Escherichia Coli.  
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Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  
Formulation: Lyophilized from (1mg/ml) solution containing 7.21 mg sodium chloride and 0.77 mg ammonium acetate per ml. 
Solubility: It is recommended to reconstitute the lyophilized Interferon-alpha 2A in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability: Lyophilized Interferon alpha 2A although stable sterile at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2A should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. 
Purity: Greater than 98.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-alpha. The N-terminal methionine has been completely removed enzymatically. 
Dimers and Aggregates: Over 95% monomers 
Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 10E8 IU/mg. 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of INF-alpha 2A 
Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2A as a Reference Standard. 
Usage: PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household  
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