Protein Laboratories Rehovot Ltd.
October 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human interferon-alpha 2B - Cat. no. CYT-15
Description: Interferon-alpha 2B (IFN-alpha 2B) is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
Human recombinant interferon-alpha 2B produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton.
The interferon-alpha 2B gene was obtained from human leukocytes.
The IFN-alpha 2B is purified by proprietary chromatographic techniques.  
Source: Escherichia Coli. 
Lot Number:  
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Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  
Formulation: Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.  
Solubility: It is recommended to reconstitute the lyophilized Interferon-alpha 2B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability: Lyophilized Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
 
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro.



 
Dimers and Aggregates: Over 94% monomers 
Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6 x 10E8 IU/ mg.
 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of INF-alpha 2B 
Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of IFN alpha 2b as a Reference Standard.
 
Usage: PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
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