Protein Laboratories Rehovot Ltd.
May 2019

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Bovine growth hormone Cat. no. GHP-27 GENE ID 280804
Description: Recombinant bovine growth hormone produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 21833 Dalton. Predicted pI=7.81. Recombinant bovine growth hormone is purified by proprietary chromatographic techniques. 
Source: E. coli 
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Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The recombinant bovine growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.0045 M NaHCO3 previously adjusted pH 8-9. 
Solubility: It is recommended to reconstitute the lyophilized recombinant bovine growth hormone in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg recombinant bovine growth hormone per ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar. 
Stability: Lyophilized recombinant bovine growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution of recombinant bovine growth hormone and filter sterilization recombinant bovine growth hormone can be stored at 4C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. 
Purity: The purity of recombinant bovine growth hormone is geater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids of recombinant bovine growth hormone was determined and was found to be Ala-Phe-Pro-Ala-Met. 
Dimers and Aggregates: Rrecombinant bovine growth hormone content of dimers is less than 5% as determined by gel filtration chromatography. 
Biological Activity: PLR’s recombinant bovine growth hormone is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. Rcombinant bovine growth hormoneis also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD). 
Endotoxin: Less than 0.05 ng/µg (IEU/µg) of recombinant bovine growth hormone 
Protein content: Protein quantitation of recombinant bovine growth hormone was carried out by two independent methods:
UV spectroscopy at 280 nm using the absorbency value of 0.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by DNAman computer analysis program of protein sequences.
Usage: PLR's recombinant bovine growth hormone can be used for research purpose only 
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