Protein Laboratories Rehovot Ltd.
November 2019

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human insulin-like growth factor 1 (hIGF-1) CYT-21 GENE ID 3479
Description: IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant Human IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7655 Dalton.
Source: E.coli 
Lot Number:  
Quantity Shipped:  
Physical Appearance: White lyophilized (freeze-dried) powder 
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.

Solubility: It is recommended to reconstitute the lyophilized hIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 
Stability: Lyophilized hIGF-I although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 hIGF-I mg/ml and up to 2 2 mg/ml and filter sterilization gsIGF-I can be stored at +4C. 
Purity: Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions. 
Dimers and Aggregates: The purified hIGF-I consists of > 97% monomers as determined by gel-filtration chromatography.
Biological Activity: hIGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose -dependent proliferation of mouse BALBC 3T3 cells is less then 1.0 ng/ml.  
Endotoxin: Endotoxin level as measured by is <0.1EU/ug. 
Protein content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.58 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences. 
Usage: This material is offered by PLR for laboratory research only.  
Date Shipped: 20.07.2010