Protein Laboratories Rehovot Ltd.
August 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human fibroblast growth factor 2 (hFGF-2) Cat. no CYT-16
Description: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. Human FGF2 is purified by proprietary chromatographic techniques. 
Source: E. coli 
Lot Number:  
Quantity Shipped:  
Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The protein was lyophilized from a concentrated (0.5 mg/ml) solution of PBS.  
Solubility: It is recommended to reconstitute the lyophilized hFGF-2 in water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.  
Stability: Lyophilized hFGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization hFGF-2 should be aliquoted and frozen. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. 
Purity: Greater than 98.0% as determined by:
(a) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

 
Amino Acid Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be MPALPE
 
Dimers and Aggregates: Less than 10% dimers as determined by gel filtration chromatography and no agregates. 
Biological Activity: The was determined by the dose-dependant proliferation of mouse BALB/c 3T3 cells  
Endotoxin: Less than 0.05 ng/µg (0.5 EU/µg) of hFGF-2.

 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.94 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences. 
Usage: For research only 
Date Shipped: 28.03.2012