Protein Laboratories Rehovot Ltd.
March 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Bovine placental lactogen - Cat no. GHP-20
Description: Bovine recombinant placental lactogen, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques . Bovine recombinant placental lactogen is also capable of activating human and other heterologous growth hormone receptors but not ruminant GH receptors. 
Source: E. coli
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Physical Appearance: White lyophylized powder 
Formulation: Bovine recombinant placental lactogen was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.

Solubility: It is recommended to reconstitute the lyophilized bovine recombinant placental lactogen in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. 
Stability: Lyophilized bovine recombinant placental lactogen although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution of bovine recombinant placental lactogen at > 0.1 mg/ml and up to 4 mg/ml and filter sterilization bovine recombinant placental lactogen can be stored at 4C for several weeks.  
Purity: The purity of bovine recombinant placental lactogen is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel. 
Amino Acid Sequence: The sequence of the first six N-terminal amino acids of bovine recombinant placental lactogen was determined and was found to be Ala-Glu-Asp-Tyr-Ala-Pro. 
Dimers and Aggregates: The purified bovine recombinant placental lactogen (23K) consists of > 99% monomers as determined by gel-filtration chromatography.
Biological Activity: PLR’s bovine recombinant placental lactogen is fully biologically active as evidenced by inducing proliferation of Nb2 cells. 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of bovine recombinant placental lactogen  
Protein content: Protein quantitation of bovine recombinant placental lactogen was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.86 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences. 
Usage: The bovine recombinant placental lactogen is offered by PLR for laboratory research  
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