Protein Laboratories Rehovot Ltd.
November 2019

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human Fibroblast growth factor 21 - cat. no. CYT-24 GENE ID 26291
Description: Human FGF-21 is a secreted growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Recombinant human FGF-21 is a 19.5 kDa protein containing 182 amino acid residues. Human FGF-21 is purified by proprietary ion exchange chromatographic techniques. 
Source: E. coli 
Lot Number:  
Quantity Shipped:  
Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO3 previously adjusted pH 8.
Solubility: It is recommended to reconstitute the lyophilized human FGF-21 in 0.4% NaHCO3 or water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar. 
Stability: Lyophilized hFGF-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization hFGF-21 can be stored at 4C, pH 8 for up to 4 weeks. For long term storage and more diluted solutions (but not for in vivo experiments) it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. 
Purity: Greater than 98.0% as determined by:
(a) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 mM NaCl, pH 8.
(b) Analysis by reducing and non-reducing SDS-PAGE gel. 
Amino Acid Sequence: The sequence of the first eight N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro-Asp-Ser-Ser. 
Dimers and Aggregates: Less than 2% as determined by gel filtration chromatography.
Biological Activity: hFGF21 lowered body weight in diet-induced obese mice when delivered via mini-osmotic pumps. 
Endotoxin: Less than 0.05 ng/µg (0.5 EU/µg) of hFGF-21.
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences 
Usage: For research only 
Date Shipped: 21.03.2016