Protein Laboratories Rehovot Ltd.
June 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Mouse growth hormone soluble receptor (binding protein) Cat. no. ECD-21 NEW
Description: Recombinant mGHR-ECD (mGHBP), one polypeptide chain containing 247 amino acids of the extracellular domain of GH receptor and having a molecular mass of ~ 28 kDa, was purified by proprietary chromatographic techniques, similar to that reported for hGHR-ECD (see Gertler et al. J Biol Chem. (1996) 271, 24482-91). 
Source: E. coli 
Lot Number:  
Quantity Shipped:  
Physical Appearance: White lyophylized powder 
Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.5 mg/ml of NaHCO3.  
Solubility: It is recommended to reconstitute the lyophilized mGHR-ECD in sterile 0.4% NaHCO3 adjusted to pH 8 or in distilled water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.  
Stability: Lyophilized mGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution at > 0.1 mGHR-ECD mg/ml and filter sterilization hGHBP can be stored at 4C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. 
Purity: Greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser. 
Dimers and Aggregates: The purified mGHR-ECD (28K) consists of > 95% monomers as determined by gel-filtration chromatography.
 
Biological Activity: PLR’s mGHR-ECD is fully biologically active as evidenced by its ability of forming 2:1 complex with human and non-primate GHs. 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rbGHBP (28 K). 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.37 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).  
Usage: This material is offered by PLR for laboratory research 
Date Shipped: 06.08.2016