Protein Laboratories Rehovot Ltd.
January 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human prolactin antagonist - Cat. no. GHP-40 NEW
Description: Recombinant human prolactin, del 1-9 G129R mutant consists of one polypeptide chain containing 190 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 22 kDa, was purified by proprietary chromatographic techniques as described by Oclon et al. PEDS (in press) 
Source: E. coli 
Lot Number:  
Quantity Shipped:  
Physical Appearance: Lyophylized white powder 
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.

 
Solubility: It is recommended to reconstitute the lyophilized hPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.



 
Stability: Lyophilized del 1-9 G129R hPRL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 hPRL mg/ml and up to 3 mg/ml and filter sterilization hPRL can be stored at 4C for several weeks.
 
Purity: Greater than 97.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
 
Amino Acid Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Arg-Ser-Gln-Val-Thr
 
Dimers and Aggregates: The purified del 1-9 G129R hPRL consists of > 95% monomers as determined by gel-filtration chromatography.



 
Biological Activity: PLR’s del 1-9 G129R hPRL is fully biologically active as evidenced by inhibiting hPRL-induced proliferation of Nb2 cells or Baf3 cells stably transfected with hPRL receptors. It also interacts at 1:1 molar ratio with human prolactin receptor extracellular domain as documanted by SEC and SPR (Biacore analysisi).  
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of del 1-9 G129R hPRL  
Protein content: Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.96 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by theDNAman computer analysis program of protein sequences. 
Usage: This material is offered by PLR for laboratory research  
Date Shipped: 24.11.2017