Protein Laboratories Rehovot Ltd.
November 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human Interleukin 2 - hIL2 (Cat no. CYT 25) NEW
Description: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Interleukin-2 human recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15.4kDa. Human IL-2 is purified by proprietary chromatographic techniques.

 
Source: E. coli 
Lot Number:  
Quantity Shipped:  
Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The protein was lyophilized from a concentrated (1.0 mg/ml) solution containing 0.05% of NaHCO3. and glycine.

 
Solubility: Lyophilized Interleukin 2 although stable at room temperature for at least 2 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored sterile at 4°C between 7-14 days and for future use below -18°C.
Please avoid freeze-thaw cycles.

 
Stability: It is recommended to reconstitute the lyophilized hIL 2 in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein such as BSA or HSA.
 
Purity: Purity: Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Ser-Ser 
Dimers and Aggregates: The purified hIL 2 consists of > 95% monomers as determined by gel-filtration chromatography under non reducing conditions.




 
Biological Activity: PLR’s hIL 2 is fully biologically active as evidenced by its ability to induce proliferation of Nb2-11C cells.

 
Endotoxin: Less than 0.05 ng/µg (0.5 IEU/µg) of hIL 2 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences  
Usage: This material is offered by PLR for laboratory research ONLY 
Date Shipped: 03.07.2018