Protein Laboratories Rehovot Ltd.
December 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Bovine leptin - Cat no. LEP-1
Description: Recombinant bovine leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Bovine leptin was purified by proprietary chromatographic techniques, see Raver et al. Prot. Express. Purif. 19, 30 -40 (2000). 
Source: E. coli 
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Physical Appearance: iWhite lyophilized (freeze-dried) powder 
Formulation: The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. 
Solubility: It is recommended to reconstitute the lyophilized recombinant bovine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.  
Stability: Lyophilized bovine leptin although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization bovine leptin can be stored at +4C. 
Purity: The recombinant bovine leptin purity is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Gel-filtration chromatography under non denaturing conditions. 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids of recombinant bovine leptin was determined and was found to be Ala-Val-Pro-Ile-Arg
 
Dimers and Aggregates: The purified recombinant bovine leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography. 
Biological Activity: PLR’s recombinant bovine leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. 
Endotoxin: The endotoxin comtent of Less than 0.1 ng/µg (IEU/µg) of recombinant bovine leptinrecombinant  
Protein content: Protein quantitation of recombinant bovine leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) of bovine leptin in solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
Usage: Recombinant bovine leptin is offered by PLR for laboratory research 
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