| Bovine leptin - Cat no. LEP-1 | |
| Description: | Recombinant bovine leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Bovine leptin was purified by proprietary chromatographic techniques, see Raver et al. Prot. Express. Purif. 19, 30 -40 (2000). |
| Source: | E. coli |
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| Physical Appearance: | iWhite lyophilized (freeze-dried) powder |
| Formulation: | The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized recombinant bovine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized bovine leptin although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization bovine leptin can be stored at +4C. |
| Purity: | The recombinant bovine leptin purity is greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) Gel-filtration chromatography under non denaturing conditions. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids of recombinant bovine leptin was determined and was found to be Ala-Val-Pro-Ile-Arg |
| Dimers and Aggregates: | The purified recombinant bovine leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography. |
| Biological Activity: | PLR’s recombinant bovine leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
| Endotoxin: | The endotoxin comtent of Less than 0.1 ng/µg (IEU/µg) of recombinant bovine leptinrecombinant |
| Protein content: | Protein quantitation of recombinant bovine leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) of bovine leptin in solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Usage: | Recombinant bovine leptin is offered by PLR for laboratory research |
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