| Mouse leptin - Cat no. LEP-6 | |
| Description: | Recombinant mouse leptin, one polypeptide chain containing 146 amino acids and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Recombinant mouse leptin was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purication 47, 128–136 |
| Source: | E. coli |
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| Physical Appearance: | White lyophilized (freeze-dried) powder. |
| Formulation: | The recombinan protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Solubility: | It is recommended to reconstitute the lyophilized recombinant mouse leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. |
| Stability: | Lyophilized recombinant mouse leptin although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 recombinant mouse leptin mg/ml and up to 2 mg/ml and filter sterilization hLEP can be stored at +4C. |
| Purity: | The purity of recombinant mouse leptin is greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids of recombinant mouse leptin was determined and was found to be Ala-Val-Pro-Ile-Gln. |
| Dimers and Aggregates: | The purified recombinant mouse leptin (16K) consists of > 93% monomers as determined by gel-filtration chromatography. |
| Biological Activity: | PLR’s recombinant mouse leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
| Endotoxin: | Less than 0.1 ng/µg (IEU/µg) of mouse leptin |
| Protein content: | Protein quantitation of recombinant mouse leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Usage: | Recombinant mouse leptin is offered by PLR for laboratory research |
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