Protein Laboratories Rehovot Ltd.
August 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human brain derived neurothropic factor (hBDNF) - Cat no. CYT-1
Description: BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions.

 
Source: E.coli
 
Lot Number:  
Quantity Shipped:  
Physical Appearance: White lyophilized (freeze-dried) powder.  
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.




 
Solubility: It is recommended to reconstitute the lyophilized hBDNF in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
 
Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20C. Reconstituted human BDNF should be stored in working aliquots at -20C.
 
Purity: Greater than 98% by SDS-PAGE and FPLC analyses.
 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ser-Asp-Pro

 
Dimers and Aggregates: Over 95% dimer under non denaturing conditions 
Biological Activity: Determined by its ability to stimulate chick E8 neurite outgrowth. The expected ED50 for this effect is 0.1-1.0 ng/ml.
 
Endotoxin: Endotoxin level is less than 0.1 ng per µg (1EU/µg). 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbance value of 1.68 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman program.

 
Usage: This material is offered by PLR for laboratory research ONLY 
Date Shipped: