Protein Laboratories Rehovot Ltd.
November 2019

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human interleukin 10 (hIL 10) - Cat no. CYT-8 GENE ID 3586
Description: IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in inhibition of immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Interleukin-10 Human Recombinant produced in E. coli is a non covalent dimer of two polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton.
The IL-10 is purified by proprietary chromatographic techniques. 
Source: E. coli

Lot Number:  
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Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.02-0.03% of NaHCO3 and glycine. 
Solubility: Lyophilized Interleukin 10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored sterile at 4°C between 7-14 days and for future use below -18°C.
Please avoid freeze-thaw cycles. 
Stability: It is recommended to reconstitute the lyophilized hIL 10 in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein such as BSA or HSA.
Purity: Purity: Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel. 
Amino Acid Sequence: The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Ser-Pro-Gly-Gln.  
Dimers and Aggregates: The purified hIL 10 consists of > 99% dimers as determined by gel-filtration chromatography under non reducing conditions.
Biological Activity: PLR’s hIL 10 is fully biologically active as evidenced by its ability to induce proliferation of IM/9 cells in presence of IL 3.

Endotoxin: Less than 0.05 ng/µg (0.5 IEU/µg) of hIL 10 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.46 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
Usage: This material is offered by PLR for laboratory research  
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