Protein Laboratories Rehovot Ltd.
May 2019

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human growth hormone soluble receptor (so called hGH binding protein) - Cat no. ECD-1 GENE ID 2690
Description: Recombinant hGHBP, one polypeptide chain containing 248 amino acids and having a molecular mass of ~ 28 kDa, hGHBP was purified by proprietary chromatographic techniques, (see Gertler et al. J Biol Chem. (1996) 271, 24482-91).

Source: Escherichia coli.

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Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The protein was lyophilized from a concentrated (0.3 mg/ml) solution in Tris-HCl, pH 8 + 300 mM NaHCO3 and 10-fold excess (w/w)of glycine. 
Solubility: It is recommended to reconstitute the lyophilized hGHBP in sterile 0.4% NaHCO3 adjusted to pH 8 or in ditilled water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.  
Stability: Lyophilized hGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 hGHBP mg/ml and filter sterilization hGHBP can be stored at 4C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. 
Purity: Greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.
Dimers and Aggregates: The purified hGHBP (28K) consists of > 98% monomers as determined by gel-filtration chromatography. 
Biological Activity: PLR’s hGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with hGH. 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of hGHBP (28 K). 
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).  
Usage: This material is offered by PLR for laboratory research  
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