Protein Laboratories Rehovot Ltd.
August 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Fish, common carp (Cyprinus carpio) growth hormone - Cat no. GHP-7
Description: Recombinant common carp (Cyprinus carpio) growth hormone (caGH) produced in E.Coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of 21404 Da. Recombinant common carp growth hormone is purified by chromatographic techniques, according to Fine et al. General and Comparative Endocrinology, 89,51-61 (1993). 
Source: E. coli
 
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Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The recombinant common carp growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.03% NaHCO3 adjusted to pH 8. 
Solubility: It is recommended to reconstitute the lyophilized recombinant common carp growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml. Recombinant common carp growth hormone can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. 
Stability: Lyophilized recombinant common carp growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution of recombinant common carp growth hormone at > 0.1 mg/ml and filter sterilization can be stored at 4C for at least two weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.  
Purity: The purity od recombinant common carp growth hormone is greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC. 
Amino Acid Sequence: The sequence of the first seven N-terminal amino acids of recombinant common carp growth hormone was determined and was found to be Ser-Asp-Asn-Gln-Arg-Leu-Phe. 
Dimers and Aggregates: The purified recombinant common carp growth hormone (21.4K) consists of > 98% monomers as determined by gel-filtration chromatography. 
Biological Activity: Recombinant common carp growth hormone is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent in vivo in promoting carp growth (Fine et al.1993). Recombinant common carp growth hormone is capable to form a 1:2 complex with oGHR-ECD and induce proliferation of PFD-P1 cells stably transfected with rabbit growth hormone receptor, though its activity is 5-fold lower compared to bovine GH (A. Gertler, unpublished data). 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of recombinant common carp growth hormone.  
Protein content: Protein quantitation of recombinant common carp growth hormone was carried out by two independent methods:
UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by DNAman computer analysis program of protein sequences. 
Usage: The recombinant common carp growth hormone is offered by PLR for research, laboratory or further manufacturing purposes.  
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