Protein Laboratories Rehovot Ltd.
June 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human leptin binding domain - Cat no. LBD-2
Description: Recombinant human leptin binding domain (hLBD), is one polypeptide chain containing 208 amino acids and an additional Ala at N-terminus acids. It consists of the cytokine binding domain of leptin receptor (amino acids 428-635 of human leptin receptor and having a molecular mass of ~ 24.5 kDa, It was purified by proprietary chromatographic techniques (see Sandowski et al. J. Biol. Chem. (2002) 277(48):46304-9.  
Source: E. coli  
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Physical Appearance: White lyophilized (freeze-dried) powder  
Formulation: The protein was lyophilized from a concentrated (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150 mM NaCl and 10-fold (w/w) excess of glycine. This formulation allows full solubilization by adding water and gentle mixing and fully preserves the binding capacity of hLBD. 
Solubility: It is recommended to reconstitute the lyophilized hLBD in sterile water adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. 
Stability: Lyophilized hLBD should be stored desiccated below -18C. Upon reconstitution at > 0.1 hLBD mg/ml and up to 40.5 mg/ml mM and filter sterilization hLBD can be stored at 4C for several months. Sterile solutions at 0.5 mg/ml or less are stable at +4C for several months. 
Purity: Purity: Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel. 
Amino Acid Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile 
Dimers and Aggregates: The purified hLBD (24K) consists of > 95% monomers as determined by gel-filtration chromatography.  
Biological Activity: PLR’s hLBDL is fully biologically active as evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.  
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of hLBD  
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 9.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
 
Usage: This material is offered by PLR for laboratory research  
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