Protein Laboratories Rehovot Ltd.
August 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) - Cat no. CYT-4
Description: Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL-6 , IL-7 , EPO and the Prolactin receptors . is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. Recombinant GM-CSF is purified by proprietary chromatographic techniques. 
Source: Recombinant Human Granulocyte Macrophage CSF is produced in E.Coli. 
Lot Number:  
Quantity Shipped:  
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  
Formulation: GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4  
Solubility: It is recommended to reconstitute the lyophilized GM-CSF in sterile DD endotoxin free water less than 1000µg/ml, which can then be further diluted to other aqueous solutions contaning neutral protein as a carrier.  
Stability: Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be sterilized and stored at 4 C up to 7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE
(d) gel filtration on Superdex 75 column. 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.  
Dimers and Aggregates: Less than 1% as determined by gel filtration on Superdex column under non denturative conditions. 
Biological Activity: PLR's GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1 ng/ml, corresponding to a Specific Activity of 11.1x106 IU/mg.  
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of GM-CSF .  
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.97 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences. 
Usage: This material is offered by PLR for laboratory research  
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