Protein Laboratories Rehovot Ltd.
August 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human placental growth hormone 20K - Cat no. GHP-12
Description: Recombinant Placental Human GH-20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. rHuGH-20K-pl is almost completely devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary hGHs.
rHuGH-20K-pl is purified by proprietary chromatographic techniques.
 
Source: Escherichia coli. 
Lot Number:  
Quantity Shipped:  
Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder. 
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045 M NaHCO3 previously adjusted pH 11. 
Solubility: It is recommended to reconstitute the lyophilized rHuGH-20K-pl in 0.4% NaHCO3 or water adjusted to pH 11, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.  
Stability: Lyophilized rHuGH-20K-pl although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization rHuGH-20K-pl can be stored at 4C, pH 11 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. 
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 11.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile. 
Dimers and Aggregates: Less than 3% as determined by silver-stained SDS-PAGE gel analysis.  
Biological Activity: PLR’r HuGH-20K is fully biologically active when compared to World Health Organization (WHO) reference standard as evidenced by in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant human growth hormone receptor extracellular domain (ECD). 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of human GH. 
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by DNAman computer analysis program of protein sequences.  
Usage: This material is offered by PLR for research, laboratory or further manufacturing purposes. 
Date Shipped: