Protein Laboratories Rehovot Ltd.
March 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human interleukin 7 (IL-7) - Cat no. CYT-7
Description: Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis since it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients.
Recombinant Human IL-7 is a single, non-glycosylated polypeptide chain containing 172 amino acids and having a molecular mass of 17412 Dalton.

Human IL-7 is purified by proprietary chromatographic techniques.

Source: Recombinant Human IL-7 produced in E.Coli  
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Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility: It is recommended to reconstitute the lyophilized rHuIL-7 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuIL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-7 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Cys-Asp-Ile.

Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis and gel filtration.
Biological Activity: PLR's rHuIL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is less then 0.5ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg.

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuIL-7.  
Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
Usage: PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.  
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