Protein Laboratories Rehovot Ltd.
January 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Chicken growth hormone (chGH) Cat no GHP-1
Description: Chicken recombinant growth hormone (chGH) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22237 Dalton. Chicken recombinant growth hormone is purified by proprietary chromatographic techniques. For reference see Eliasiewicz et al. (2006) Ann N Y Acad Sci. 1091:501-508.  
Source: E. coli 
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Physical Appearance: White lyophilized (freeze-dried) powder. 
Formulation: The chicken recombinant growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8. 
Solubility: It is recommended to reconstitute the lyophilized chicken recombinant growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. 
Stability: Lyophilized chicken recombinant growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution of chicken recombinant growth hormone at > 0.1 mg/ml and up to 3 mg/ml and filter sterilization chicken recombinant growth hormone can be stored at 4C for several weeks. At lower concentration of chicken recombinant growth hormone addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.  
Purity: The purity of chicken recombinant growth hormone is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Analysis by RP-HPLC. 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids of chicken recombinant growth hormone was determined and was found to be Ala-Thr-Phe-Pro-Ala. 
Dimers and Aggregates: The purified chicken recombinant growth hormone (22K) consists of > 99% monomers as determined by gel-filtration chromatography. 
Biological Activity: Chicken recombinant growth hormone is fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit growth hormonr receptors. 
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of chicken recombinant growth hormone. 
Protein content: Protein quantitation of chicken recombinant growth hormone was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the DNAman program.  
Usage: The chicken recombinant growth hormone is offered by PLR for research, laboratory or further manufacturing purposes. 
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