| Chicken growth hormone (chGH) Cat no GHP-1 | |
| Description: | Chicken recombinant growth hormone (chGH) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22237 Dalton. Chicken recombinant growth hormone is purified by proprietary chromatographic techniques. For reference see Eliasiewicz et al. (2006) Ann N Y Acad Sci. 1091:501-508. |
| Source: | E. coli |
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| Physical Appearance: | White lyophilized (freeze-dried) powder. |
| Formulation: | The chicken recombinant growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8. |
| Solubility: | It is recommended to reconstitute the lyophilized chicken recombinant growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. |
| Stability: | Lyophilized chicken recombinant growth hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution of chicken recombinant growth hormone at > 0.1 mg/ml and up to 3 mg/ml and filter sterilization chicken recombinant growth hormone can be stored at 4C for several weeks. At lower concentration of chicken recombinant growth hormone addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles. |
| Purity: | The purity of chicken recombinant growth hormone is greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) Analysis by RP-HPLC. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids of chicken recombinant growth hormone was determined and was found to be Ala-Thr-Phe-Pro-Ala. |
| Dimers and Aggregates: | The purified chicken recombinant growth hormone (22K) consists of > 99% monomers as determined by gel-filtration chromatography. |
| Biological Activity: | Chicken recombinant growth hormone is fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit growth hormonr receptors. |
| Endotoxin: | Less than 0.1 ng/µg (IEU/µg) of chicken recombinant growth hormone. |
| Protein content: | Protein quantitation of chicken recombinant growth hormone was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the DNAman program. |
| Usage: | The chicken recombinant growth hormone is offered by PLR for research, laboratory or further manufacturing purposes. |
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