Protein Laboratories Rehovot Ltd.
March 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Human interleukin 3 (IL-3) - Cat no. CYT-6
Description: Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases
Recombinant Human IL-3 is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton.

Source: Recombinant Human IL-3 produced in E.Coli.
The rHuIL-3 is purified by proprietary chromatographic techniques.  
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Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.  
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.
Solubility: It is recommended to reconstitute the lyophilized rHuIL-3 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuIL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-3 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration Chromatoigraphy
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.
Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: PLR's rHuIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuIL-3.  
Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
Usage: PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
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