Protein Laboratories Rehovot Ltd.
August 2018

Certificate of Analysis & Data Sheet

We are the ONLY company producing leptin antagonists and leptins of farm animals including several fish species.

Pegylated human, mouse, rat and ovine leptins and corresponding leptin antagonists are now available!!!

PLR provides a novel service of pegylation of either existing, or provided or custom-made proteins. All PLR's proteins are carrier free (cf) and can be sold in any requested amount !!!
Dog Leptin - Cat no. LEP-3
Description: Recombinant dog leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Dog leptin was purified by proprietary chromatographic techniques, (Solomon, Charlier, Djiane and Gertler, unpublished results). 
Source: E. coli.
 
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Physical Appearance: White lyophilized (freeze-dried) powder 
Formulation: The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
 
Solubility: It is recommended to reconstitute the lyophilized recombinant dog leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 
Stability: Lyophilized recombinant dog leptin although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg of dog leptin and filter sterilization recombinant dog leptin can be stored at +4C. 
Purity: The purity of recombinant dog leptin is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Gel-filtration chromatography under non denaturing conditions. 
Amino Acid Sequence: The sequence of the first five N-terminal amino acids of recombinant dog leptin was determined and was found to be Ala-Val-Pro-Ile-Arg  
Dimers and Aggregates: The purified recombinant dog leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography.
 
Biological Activity: PLR’s recombinant dog leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. 
Endotoxin: The endotoxin content of recombinant dog leptin is less than 0.05 ng/µg (0.5 IEU/µg) of dog leptin 
Protein content: Protein quantitation of recombinant dog leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
Usage: Recombinant dog leptin is offered by PLR for laboratory research 
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