Rat cilliary neurothropic factor (rCNTF) - Cat no. CYT-82 GENE ID 25708

CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant rat CNTF is synthesized as a 200 amino acid polypeptide (22834 Da) lacking a hydrophobic N-terminal signal for secretion.

E. coli

 

 

White lyophilized (freeze-dried) powder.

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.

It is recommended to reconstitute the lyophilized rCNTF in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Lyophilized rCNTF although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg/ml mM and filter sterilization rCNTF can be stored at 4C for several weeks.

Purity: Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ala-Glu-Gln.

The purified rCNTF (22.8 kDa) consists of > 99% monomers as determined by gel-filtration chromatography.

PLR’s rCNTF is fully biologically active as evidenced by its ability to phosphorylate STAT3 in several cells lines.

Less than 0.05 ng/µg (IEU/µg) of rCNTF

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.22 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (DNAman).

This material is offered by PLR for laboratory research