Description:
Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Recombinant mouse IL-1Ra is a 17.4 kDa protein consisting of 153 amino acid residues. Recombinant monomeric murine IL-1Ra is purified by proprietary chromatographic techniques.
Source:
E. coli
Lot Number:
Quantity Shipped:
Physical Appearance:
White lyophilized (freeze-dried) powder.
Formulation:
Interleukin-1Ra is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
Solubility:
It is recommended to reconstitute the lyophilized IL-1Ra in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar.
Stability:
Lyophilized IL-1Ra although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(b) Gel filtration chromatography under non-denaturing conditions.
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(b) Gel filtration chromatography under non-denaturing conditions.
Amino Acid Sequence:
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Cys-Arg-Pro-Ser-Gly.
Dimers and Aggregates:
Less than 2% as determined by gel filtration under non-denaturing conditions.
Biological Activity:
PLR’s murine IL-1Ra is capable of inhibitinf IL-1-alpha activity in helper T cell line D10.G4.1
Endotoxin:
Less than 0.05 ng/µg (0.5 EU/µg) of recombinant IL-1α.
Protein content:
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein
sequences.
sequences.
Usage:
PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Date Shipped: