Ovine placental lactogen - Cat no. GHP-18 GENE ID 443319

Description:
Recombinant ovine placental lactogen, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Sakal et al. J. Endocrinology (1997), 152, 317-327. Ovine PL is also capable of activating human and other heterologous GH receptors but not ruminat GH receptors.
Source:
E.coli
Lot Number:
 
Quantity Shipped:
 
Physical Appearance:
White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
Solubility:
It is recommended to reconstitute the lyophilized oPL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability:
Lyophilized oPL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 oPL mg/ml and up to 4 mg/ml mM and filter sterilization oPL can be stored at 4C for several weeks.
Purity:
Greater than 97.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gln-His-Pro-Pro.
Dimers and Aggregates:
The purified oPL (23K) consists of > 95% monomers as determined by gel-filtration chromatography.
Biological Activity:
PLR’s oPL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of oPL
Protein content:
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by DNAman computer analysis program of protein sequences
Usage:
This material is offered by PLR for laboratory research
Date Shipped:
 
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