Description:
Ostreolysin, is a cytosolic prtein of 15 kDa pore-forming protein from the edible oyster mushroom (Pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. Its cytotoxicity to Chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. Moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingomyelin or dipalmitoyl-phosphatidylcholine. Addition of mono- or di-unsaturated phosphatidylcholine to the cholesterol/sphingomyelin vesicles dramatically reduces the ostreolysin's activity. It appears that the protein recognizes specifically a cholesterol-rich lipid phase, probably the liquid-ordered phase. Ostreolysin was purified by combination of ion-exchange and size-exclusion chromatography.
Source:
E. coli
Lot Number:
Quantity Shipped:
Physical Appearance:
White powder
Formulation:
Ostreolysin was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Solubility:
It is recommended to reconstitute the lyophilized ostreolysin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized ostreolysin although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 protein mg/ml and up to 2 mg and filter sterilization ostreolysin can be stored at +4C.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Amino Acid Sequence:
The N-terminal amino sequence is Ala-Tyr-Ala-Gln-Trp-Val
Dimers and Aggregates:
Less than 2%.
Biological Activity:
Ostreolysin has potent anti-carcinogenic activity in several colon cancer cell lines.
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of ostreolysin
Protein content:
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNA-man computer analysis program.
Usage:
This material is offered by PLR for laboratory research
Date Shipped: