Rat insulin-like growth factor 1 (rIGF-1) CYT-103 GENE ID 24482

IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant rat IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7.6 kDa.
E. coli
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Physical Appearance:
White powder
The protein was lyophilized with no additives.
It is recommended to reconstitute the lyophilized rIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized rIGF-I although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 rIGF-I mg/ml and up to 2 mg/ml and filter sterilization rbIGF-I can be stored at +4C.
Greater than 98.0% as determined by SDS-PAGE.
Amino Acid Sequence:
Dimers and Aggregates:
The purified rIGF-I consists of > 95% monomers as determined by gel-filtration chromatography.
Biological Activity:
The ED50, was calculated 2 methods:
1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be < 30ng/ml corresponding to a Specific Activity of 33,334IU/mg.
2. Type 1 IGF receptor binding assay ED50 was found to be <10ng/ml corresponding to a Specific Activity of 100,000IU/mg.
Endotoxin level is <0.5 EU/ug
Protein content:
Protein quantification was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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