Chicken prolactin (chPRL) - Cat. no. GHP-37 GENe ID 196660 NEW

Description:
Recombinant chickenn prolactin, (chPRL) one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques as described by Oclon et al (2017) GCE 240, 27-34.

Source:
E. coli
Lot Number:
 
Quantity Shipped:
 
Physical Appearance:
White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.

Solubility:
It is recommended to reconstitute the lyophilized chPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability:
Lyophilized chPRL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 chPRL mg/ml and up to 3 mg/ml and filter sterilization hPRL can be stored at 4C for several weeks.
Purity:
Greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

Amino Acid Sequence:
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Ile-Cys-Pro.

Dimers and Aggregates:
The purified chPRL (23K) consists of > 95% monomers as determined by gel-filtration chromatography.


Biological Activity:
PLR’s hPRL is biologically active as evidenced by inducing proliferation of Nb2 cells and baf/3 cells stably transfected with hPRL receptors and by in vivo in chicken.
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of hPRL
Protein content:
Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
Usage:
PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Date Shipped:
 
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