Horse leptin - Cat no. LEP-4 GENE ID 100034042

Recombinant horse leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant horse leptin was purified by proprietary chromatographic techniques, (unpublished).

E. coli

 

 

White lyophilized (freeze-dried) powder.

The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

It is recommended to reconstitute the lyophilized recombinant horse leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized recombinant horse leptin although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 recombinant horse leptin mg/ml and up to 2 mg and filter sterilization recombinant horse leptin can be stored at +4C.

The purity of recombinant horse leptin is greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

The sequence of the first five N-terminal amino acids of recombinant horse leptin was determined and was found to be Ala-Val-Pro-Ile-Gln

The purified racombinant horse leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography.

PLR’s recombinant horse leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

The endotoxin content of recombinant horse leptin is less than 0.1 ng/µg (IEU/µg) of horse leptin

Protein quantitation of recombinant horse leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

This recombinant horse leptin is offered by PLR for laboratory research