Description:
Recombinant pituitary human growth hormone - 20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20322 Dalton. Recombinant pituitary human growth hormone
20K is purified by proprietary chromatographic techniques (see Solomon et al. Growth Horm IGF Res. 2006;16:297-307).
20K is purified by proprietary chromatographic techniques (see Solomon et al. Growth Horm IGF Res. 2006;16:297-307).
Source:
E. coli
Lot Number:
Quantity Shipped:
Physical Appearance:
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation:
The recombinant pituitary human growth hormone 20K was lyophilized from a concentrated (1mg/ml) solution with 0.0045 M NaHCO3 previously adjusted pH 10.
Solubility:
It is recommended to reconstitute the lyophilized recombinant pituitary human growth hormone 20K in 0.4% NaHCO3 adjusted to pH 10, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized recombinant pituitary human growth hormone
20K although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization recombinant pituitary human growth hormone 20Kcan be stored at 4C, pH 10 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
20K although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization recombinant pituitary human growth hormone 20Kcan be stored at 4C, pH 10 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:
The purity of recombinant pituitary human growth hormone
20K is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 9.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
20K is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 9.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids of recombinant pituitary human growth hormone 20K was determined and was found to be Ala-Phe-Pro-Thr-Ile.
Dimers and Aggregates:
Less than 1% as determined by gel filtration chromatography of recombinant pituitary human growth hormone 20K under non denaturative conditions.
Biological Activity:
Recombinant pituitary human growth hormone 20K is fully biologically active when compared to World Health Organization (WHO) reference standard as evidenced by several in vitro bioassays. Recombinant pituitary human growth hormone 20K is also capable of forming a 1:2 complex with the recombinant human growth hormone receptor extracellular domain (ECD).
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of recombinant pituitary human growth hormone 20K.
Protein content:
Protein quantitation of recombinant pituitary human growth hormone 20Kwas carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by DNAman computer analysis program of protein sequences.
1. UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by DNAman computer analysis program of protein sequences.
Usage:
The recombinant pituitary human growth hormone 20 K is offered by PLR for research, laboratory or further manufacturing purposes.
Date Shipped: