Certificate of Analysis & Data Sheet

IL-1 is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1; and IL-1RA binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Recombinant mouse IL-1; is an 18.4 kDa protein containing 157 amino acid residues. Recombinant monomeric murine IL-1 is purified by proprietary chromatographic techniques.

E. coli

Lypohylized white powder

Interleukin-1α is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.

It is recommended to reconstitute the lyophilized IL-1 in sterile -18C in H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar.

Lyophilized IL-1-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(b) Gel filtration chromatography under non-denaturing conditions.

The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ser-Ala-Pro-Tyr-Thr.

Less than 2% as determined by gel filtration under non-denaturing conditions.

PLR’s murine IL-1 alpha is biologically active in helper T cell line D10.G4.1

Less than 0.05 ng/µg (0.5 EU/µg) of recombinant IL-1α.

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.33 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein
sequences.

PRL's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.