Certificate of Analysis & Data Sheet

IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant mouse IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7.6 kDa.

E. coli

White lyophilized (freeze-dried) powder.

The protein was lyophilized with no additives.

It is recommended to reconstitute the lyophilized IGF1 in sterile 18MΩ-cm H2O or 0.4% NaHCO3 not less than 100µg/ml, and not more than 2 mg/ml which can then be further diluted to other aqueous solutions.

Lyophilized IGFI although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF-1 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

MGPETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKAA

The purified mIGF-I consists of > 95% monomers as determined by gel-filtration chromatography.

The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1MU/mg.

Endotoxin level is <0.5 EU/ug

Protein quantification was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

PLR's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.