Certificate of Analysis & Data Sheet

Recombinant mGHR-ECD (mGHBP), one polypeptide chain containing 247 amino acids of the extracellular domain of GH receptor and having a molecular mass of ~ 28 kDa, was purified by proprietary chromatographic techniques, similar to that reported for hGHR-ECD (see Gertler et al. J Biol Chem. (1996) 271, 24482-91).

E. coli

White lyophylized powder

The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.5 mg/ml of NaHCO3.

It is recommended to reconstitute the lyophilized mGHR-ECD in sterile 0.4% NaHCO3 adjusted to pH 8 or in distilled water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized mGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution at > 0.1 mGHR-ECD mg/ml and filter sterilization hGHBP can be stored at 4C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.

The purified mGHR-ECD (28K) consists of > 95% monomers as determined by gel-filtration chromatography.

PLR’s mGHR-ECD is fully biologically active as evidenced by its ability of forming 2:1 complex with human and non-primate GHs.

Less than 0.1 ng/µg (IEU/µg) of rbGHBP (28 K).

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.37 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

This material is offered by PLR for laboratory research