Protein Laboratories Rehovot Ltd.
Human Interleukin 2 - hIL2 (Cat no. CYT 25) GENE ID 3558 NEW
Description:
Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Interleukin-2 human recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15.4kDa. Human IL-2 is purified by proprietary chromatographic techniques.
Source:
E. coli
Physical Appearance:
White lyophilized (freeze-dried) powder.
Formulation:
The protein was lyophilized from a concentrated (1.0 mg/ml) solution containing 0.05% of NaHCO3. and glycine.
Solubility:
Lyophilized Interleukin 2 although stable at room temperature for at least 2 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored sterile at 4°C between 7-14 days and for future use below -18°C.
Please avoid freeze-thaw cycles.
Please avoid freeze-thaw cycles.
Stability:
It is recommended to reconstitute the lyophilized hIL 2 in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein such as BSA or HSA.
Purity:
Purity: Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Ser-Ser
Dimers and Aggregates:
The purified hIL 2 consists of > 95% monomers as determined by gel-filtration chromatography under non reducing conditions.
Biological Activity:
PLR’s hIL 2 is fully biologically active as evidenced by its ability to induce proliferation of Nb2-11C cells.
Endotoxin:
Less than 0.05 ng/µg (0.5 IEU/µg) of hIL 2
Protein content:
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences
Usage:
This material is offered by PLR for laboratory research ONLY