Protein Laboratories Rehovot Ltd.
Human alpha synuclein cat. no. OTH-3 GENE ID 6622 NEW
Description:
Alpha-Synuclein is a member of the Synuclein family. It is expressed principally in brain but also expressed in low concentrations in all tissues except liver. Alpha synuclein interacts with UCHL1, Phospholipase D and histones. In addition, alpha synuclein is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that alpha synuclein is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in its action will lead to Dementia Lewy Body (DLB).Human Alpha-Synuclein, is also known as Non-A Beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, SNCA, NACP, PARK1. Recombinant Human alpha-Synuclein is produced by our E.coli expression system and the target gene encoding Met1-Ala140 is expressed, and purified as periplasmic protein by ion-exchange chromatography.
Source:
E. coli
Physical Appearance:
White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2 μm filtered solution of 25mM Na-acetate pH 4.2.
Solubility:
Always centrifuge tubes before opening. Dissolve the lyophilized protein in ddH2O. Mix gently and do not mix by vortex.
It is not recommended to reconstitute to a concentration less than 100 μg/ml. Aliquot the reconstituted solution to minimize freeze-thaw cycles.
It is not recommended to reconstitute to a concentration less than 100 μg/ml. Aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity:
More than 95% as determined by SDS-PAGE.
Amino Acid Sequence:
The first 10 N-terminal amino acids are: MDVFMKGLSK
Dimers and Aggregates:
Over 95 % monomer as determined by SEC at pH 3.5
Biological Activity:
Not tested
Endotoxin:
Less than 0.1 ng/µg (IEU/µg) of alpha synuclein
Protein content:
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.4 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNA-man computer analysis program.
Usage:
This material is offered by PLR for laboratory research