Certificate of Analysis & Data Sheet
CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity. Recombinant human CNTF is synthesized as a 200 amino acid polypeptide (22.7 kDa) lacking a hydrophobic N-terminal signal for secretion.
White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
It is recommended to reconstitute the lyophilized hCNTF in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Lyophilized hCNTF although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg/ml mM and filter sterilization hCNTF can be stored at 4C for several weeks.
Purity: Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence:
The sequence of the first four N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr
Dimers and Aggregates:
The purified hCNTF (22K) consists of > 99% monomers as determined by gel-filtration chromatography.
PLR’s hCNTF is fully biologically active as evidenced by its ability to phosphorylate STAT3 in several cells lines.
Less than 0.1 ng/µg (IEU/µg) of hCNTF
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.242 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
This material is offered by PLR for laboratory research