Certificate of Analysis & Data Sheet
Recombinant hGHBP, one polypeptide chain containing 248 amino acids and having a molecular mass of ~ 28 kDa, hGHBP was purified by proprietary chromatographic techniques, (see Gertler et al. J Biol Chem. (1996) 271, 24482-91).
White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (0.3 mg/ml) solution in Tris-HCl, pH 8 + 300 mM NaHCO3 and 10-fold excess (w/w)of glycine.
It is recommended to reconstitute the lyophilized hGHBP in sterile 0.4% NaHCO3 adjusted to pH 8 or in ditilled water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized hGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 hGHBP mg/ml and filter sterilization hGHBP can be stored at 4C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.
Dimers and Aggregates:
The purified hGHBP (28K) consists of > 98% monomers as determined by gel-filtration chromatography.
PLR’s hGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with hGH.
Less than 0.1 ng/µg (IEU/µg) of hGHBP (28 K).
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
This material is offered by PLR for laboratory research